Molecular Beacons for Multiplex Detection of Four Bacterial Bioterrorism Agents
نویسندگان
چکیده
منابع مشابه
Molecular beacons for multiplex detection of four bacterial bioterrorism agents.
plasmon resonance (SPR). FEMS Immunol Med Microbiol 2000;29:283–8. 7. Asai R, Nakamura C, Ikebukuro K, Karube I, Miyake J. Detection technique of asymmetric RT-PCR-based amplified single-stranded DNA and its application to biosensor for detection of mRNA for cyanobacteria, Anabaena variabilis. Biotechnol Lett 2002;24:1677–82. 8. Feriotto G, Borgatti M, Mischiati C, Bianchi N, Gambari R. Biosens...
متن کاملMultiplex detection of four pathogenic retroviruses using molecular beacons.
We describe a multiplex nucleic acid assay that identifies and determines the abundance of four different pathogenic retroviruses (HIV-1, HIV-2, and human T-lymphotrophic virus types I and II). Retroviral DNA sequences are amplified in a single, sealed tube by simultaneous PCR assays, and the resulting amplicons are detected in real time by the hybridization of four differently colored, amplico...
متن کاملMultiplex detection of single-nucleotide variations using molecular beacons.
We demonstrate that single-nucleotide differences in a DNA sequence can be detected in homogeneous assays using molecular beacons. In this method, the region surrounding the site of a sequence variation is amplified in a polymerase chain reaction and the identity of the variant nucleotide is determined by observing which of four differently colored molecular beacons binds to the amplification p...
متن کاملUse of multiplex PCR for simultaneous detection of four bacterial species in middle ear effusions.
A multiplex PCR procedure was developed for the simultaneous detection of Alloiococcus otitidis, Haemophilus influenzae, Moraxella catarrhalis, and Streptococcus pneumoniae in middle ear effusions (MEEs) from patients with chronic otitis media with effusion. The bacterial 16S rRNA gene was chosen as the target, and the procedure used one common lower primer and four species-specific upper prime...
متن کاملMolecular Detection of Bacterial Etiology of Rheumatoid Arthritis
Background and purpose: Etiology of rheumatoid arthritis is not fully recognized. The purpose of this study was to use universal and specific primers to trace bacteria in the blood and synovial fluid in patients with rheumatoid arthritis. Materials and methods: In this experimental study, a PCR method was developed to identify a wide range of bacteria in general and Staphylococcus aureus in sp...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Clinical Chemistry
سال: 2004
ISSN: 0009-9147,1530-8561
DOI: 10.1373/clinchem.2003.030767